10.4225/03/57C81DC64E72B Sebastian Furness Sebastian Furness cameron.nowell@monash.edu cameron.nowell@monash.edu TIRF_Residency_script_Cell_Furness2016.ijm Monash University 2016 tirf-microscopy G protein residency signallling Receptors and Membrane Biology 2016-09-01 12:24:44 Software https://bridges.monash.edu/articles/software/TIRF_Residency_script_Cell_Furness2016_ijm/3798480 The custom script was written to measure the time a given spot was present in the TIRF field. This is achieved by extracting the individual spots as single binary points and then measuring how many frames each one is present for before the signal decreases to background.