10.4225/03/5a1e35aab0544
David Lindsay Healy
David Lindsay
Healy
Human prolactin physiology
Monash University
2017
prolactin
2017-11-29 04:20:56
Thesis
https://bridges.monash.edu/articles/thesis/Human_prolactin_physiology/5643313
The overall aim of the work to be presented in this
thesis was to investigate the biology of the protein hormone prolactin
(PFL) in two areas of reproductive endocrinology:
hypothalamic-pituitary control of PRL secretion and the possible origin
of amniotic fluid PRL. For these studies, a reliable homologous PRL
double antibody radioimmunoassay was developed using materials supplied
by the NIAMDD. Serum PRL concentrations in 60 normal men were 12.8 ±
3.2 ng/ml (mean± 2 SO) and in 24 premenopausal women 13.6 ± 5.4 ng/ml.
Depending onthe iodination procedure used, the mean assay sensitivity
was 0.55 -0.61 ng/ml, the within-assay precision (expressed as a
coefficient of variation) was between 5.6 - 6.6 per cent and the
between-assay precision for medium pool samples was 16 - 19%. Factors
crucial to the development of a reliable assay were the use of minimal
oxidation conditions for PRL iodination, complete separation of the
iodinated species so formed, use of a highly dilute PRL antiserum to
increase assay sensitivity, careful titration of the amount of
non-immune serum used to increase ant genic bulk for the second antibody
step and a long incubation time to ensure maximal immunoprecipitation. [...]