10.26180/5d9d61ec9b050
ANDREW NNALUE OSAHOR
ANDREW NNALUE
OSAHOR
Synergy of Invasive Escherichia coli and Cationic Lipids for Gene Delivery to Human Cells: Genetic Supplementation of ⍺-Galactosidase A Deficiency in Fabry Disease
Monash University
2019
Escherichia coli
Bactofection
Cell
DNA
Fabry
Invasion
Lipid
Antimicrobial
Integrin
Migfilin
Artificial chromosome
Microbiology
Cell Biology
Molecular Biology
2019-10-09 04:28:27
Thesis
https://bridges.monash.edu/articles/thesis/Synergy_of_Invasive_Escherichia_coli_and_Cationic_Lipids_for_Gene_Delivery_to_Human_Cells_Genetic_Supplementation_of_-Galactosidase_A_Deficiency_in_Fabry_Disease/9932396
Human genetic diseases can potentially be treated by replacing damaged or defective DNA. It is however extraordinarily difficult to introduce foreign DNA into living organisms. My project focused on developing a well-known bacteria species called Escherichia coli (E. coli) to efficiently deliver engineered DNA into human cells. I tested combining E. coli with reagents called lipids, which are naturally attracted to cells. These lipids were able to significantly improve the ability of E. coli to deliver therapeutic DNA; providing insights into the potential for the use of bacteria in molecular therapy.