Development of one-step fluorescent biosensors by exploiting engineered antibodies

Fluorescence-based biosensors are emerging as an alternative to traditional immunoassays. Most protein biomarkers are currently detected by assays that require multiple steps for washing, blocking and addition of secondary reagents. Hence, they are time-consuming, laborious, and require sophisticated laboratory infrastructure and trained professionals. In this PhD project, a novel one-step fluorescent biosensor was developed by exploiting engineered antibodies that rely on fluorescence modulation due to selective target binding, allowing quantification of biomarkers without the need of further reagents and sample processing.